Direct analysis of Peucedanum palustre samples by desorption atmospheric pressure photoionization-mass spectrometry |
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| Institution: | 1. Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014, University of Helsinki, Finland;2. Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, P.O. Box 56, FI-00014, University of Helsinki, Finland;1. Department of Pharmaceutical Botany and Plant Biotechnology, Poznań University of Medical Sciences, Św. Marii Magdaleny 14, 61-861 Poznań, Poland;2. Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland;3. Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland;4. Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Z. Noskowskiego 12/14, 61-704 Poznań, Poland;5. Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland;6. Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland;7. Department of Botany, Breeding and Agricultural Technology for Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland;8. Division of Perinatology and Women’s Diseases, Poznań University of Medical Sciences, Polna 33, 60-535 Poznań, Poland;9. Laboratory of Molecular Biology, Poznań University of Medical Sciences, Polna 33, 60-535 Poznań, Poland;1. Department of Chemistry, University of Botswana, Private Bag 0022, Gaborone, Botswana;2. Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Str. 6, D-44221 Dortmund, Germany;3. Department of Chemistry, University of Kinshasa, P.O. Box: 190 Kinshasa XI, Congo |
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| Abstract: | Desorption atmospheric pressure photoionization (DAPPI) is an ambient mass spectrometry (MS) technique that can be used for the analysis of polar and nonpolar compounds directly from surfaces. Here, the feasibility of DAPPI-MS in the screening of plant metabolites from dried Peucedanum palustre leaves and umbels was studied. DAPPI-MS requires no prior sample preparation or chromatographic separation, and the analysis can therefore be performed directly from the untreated plant material. P. palustre contains several linear and angular furanocoumarins, some of which are specific for the species. The DAPPI mass spectra of both leaf and umbel samples showed distinct ions at m/z 445 and 443 in positive and negative ion modes, respectively. MS2 analyses of these ions confirmed that the ions were the protonated and deprotonated molecules, respectively, of peulustrin and its isomers, which have only been identified from P. palustre. The direct analysis of dried plant material by DAPPI-MS was shown to provide a fast and reliable means to confirm the identity of plant materials, to study the metabolite profiles of plants, and to screen biologically relevant compounds from plant surfaces. |
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| Keywords: | Desorption atmospheric pressure photoionization-mass spectrometry Milk parsley Furanocoumarin Peulustrin |
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