Fractionation of UV-B absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography |
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| Affiliation: | 1. Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France;2. ARMEN Instruments, 16 Rue Ampère, 56890 Saint-Avé, France;1. Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street 1, 11566 Cairo, Egypt;2. Department of Aquaculture, Faculty of Fisheries, Izmir Katip Celebi University, Izmir 35100 Bornova, Turkey;3. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt;4. Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, Universitätsstrasse 1, Geb. 26.23, D-40225 Düsseldorf, Germany;1. Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche OENOLOGIE, 210, chemin de Leysotte, F-33882 Villenave d''Ornon, France;2. INRA, ISVV, USC 1366 OENOLOGIE, 33882 Villenave d''Ornon, France;3. Univ. de Bordeaux, ISVV, GESVAB, EA 3675, F-33882 Villenave d''Ornon, France;1. Univ. Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, Equipe Molécules d’Intérêt Biologique (Gesvab) - ISVV, 33882, Villenave d’Ornon cedex, France;2. Polyphénols Biotech (Technology Transfer Unit), ISVV, 33882, Villenave d’Ornon cedex, France |
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| Abstract: | Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310 nm. |
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| Keywords: | Rhodophyceae Centrifugal partition Chromatography UVB compounds Antioxidant |
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