Analysis of an immunoadjuvant saponin fraction from Quillaja brasiliensis leaves by electrospray ionization ion trap multiple-stage mass spectrometry |
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| Affiliation: | 1. Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR. Ruta 5 Km 386, Tacuarembó 45000, Uruguay;2. Programa Nacional de Investigación en Producción Forestal, Instituto Nacional de Investigación Agropecuaria, Ruta 5 Km 386, Tacuarembó 45000, Uruguay;3. Departamento de Química Orgánica, Facultad de Química, Laboratorio de Carbohidratos y Glicoconjugados, Udelar. Instituto de Higiene, Av. Alfredo Navarro 3051, Montevideo 11600, Uruguay;1. University of Hohenheim, Institute of Food Science and Biotechnology, Department of Food Microbiology and Hygiene, Garbenstrasse 28, 70599 Stuttgart, Germany;2. University of Hohenheim, Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology and Analysis, Garbenstrasse 25, 70599 Stuttgart, Germany;3. King Abdulaziz University, Faculty of Science, Biological Science Department, P.O. Box 80257, Jeddah 21589, Saudi Arabia;1. Department of Pediatrics, Center for Immunity and Inflammation, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ 07101, USA;1. Institute of Analytical Chemistry, Johannes-Kepler-University, Altenbergerstrasse 69, A-4040 Linz, Austria;2. Institute of Organic Chemistry, Johannes-Kepler-University, Linz, Austria;1. CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China;2. Tibet Autonomous Region Institute for Food and Drug control, Lhasa 850000, PR China;3. Syngenta Jealott’s Hill International Research Centre, Berkshire RG42 6EY, UK;1. Universidad Autónoma de Madrid, Departamento de Química Orgánica, Cantoblanco, 28049 Madrid, Spain;2. Laboratory of Photonics and Interfaces, Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), Station 6, CH 1015 Lausanne, Switzerland;3. Instituto Madrileño de Estudios Avanzados (IMDEA)-Nanociencia, c/Faraday, 9, Cantoblanco, 28049 Madrid, Spain |
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| Abstract: | An immunoadjuvant saponin fraction from Quillaja brasiliensis leaves was investigated by direct infusion and liquid chromatography/electrospray ionization ion trap multiple-stage mass spectrometry in negative ion mode (DI-ESI-IT-MSn and LC-ESI-IT-MSn). The aglycone and the sequence of the oligosaccharide residues at C-3 and C-28 were characterized based on MS2 and MS3 experiments of the [M H]− ions. According to their [MH]− ions, characteristic product ions and retention times, 27 bidesmosidic saponins, bearing four types of triterpenic aglycones, were tentatively identified. |
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| Keywords: | Triterpenic saponins Immunoadjuvant saponin fraction ESI-MS UHPLC |
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