New saikosaponins from the roots of Bupleurum chinense |
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| Institution: | 1. Faculty of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650050, China;2. College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming 650050, China;1. Department of Variety Examination, National Forest Seed and Variety Center, Chungju, Chungbuk 380-941, South Korea;2. Herbal Medicine Research Division, Herbal Research Ministry of Food and Drug Safety, Cheongju, Chungbuk 363-700, South Korea;3. Department of Biology, Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea;1. Department of Chemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey;2. Department of Chemistry, Faculty of Science, Çank?r? Karatekin University, Çank?r?, Turkey;1. Pharmacy College, Jiangxi University of Traditional Chinese Medicine, 818 Xinwan Avenue, Nanchang 330004, Jiangxi, PR China;2. Technology Center, Jiangzhong Group, 618 Huoju Road, Nanchang 330096, Jiangxi, PR China;3. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China;1. The Experimental Forest Management Office, National Chung Hsing University, Taichung 402, Taiwan;2. Department of Nursing, Hungkuang University, Taichung 443, Taiwan;3. Department of Biotechnology, Hungkuang University, Taichung 443, Taiwan;4. Department of Forestry, National Chung Hsiung University, Taichung 402, Taiwan;5. Graduate Institute of Pharmaceutical Science and Technology, Central Taiwan University of Science University, Taichung 406, Taiwan;6. Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan;7. Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan;8. Department of Chemistry, National Taiwan University, Taipei 106, Taiwan;9. Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan;10. Department of Biotechnology, Asia University, Taichung 413, Taiwan |
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| Abstract: | Seven new saikosaponins (1 ? 7), together with four known ones (8 ? 11), were isolated from the roots of Bupleurum chinense. Their structures were elucidated by comprehensive spectroscopic analysis and chemical methods. All obtained saikosaponins were oleanane-type, except for compound 11, which was the first lupane–type saikosaponin isolated from the genus Bupleurum. Compound 1 represented the first example of saikosaponins bearing a hydroxyl group at C-15, while compound 4 was a saikosaponin containing acetoxy group at C-23 rarely found in genus Bupleurum. Compounds 6 and 7, bearing an n-butoxy moiety at C-11, were confirmed to be artifacts by LC–MS experiment. All the isolates were evaluated for their inhibitory effects towards nitric oxide production induced by lipopolysaccharide in BV-2 microglial cells, and saikosaponins A (9) and D (10) exhibited the most potent activity, which were comparable to the positive control dexamethasone. |
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| Keywords: | Bupleuri Radix Saikosaponins Anti-inflammatory activity |
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