基于生物阻抗谱的生物细胞活性免标记检测方法

目的 基于生物阻抗谱（bioelectrical impedance spectroscopy，BIS）技术，提出一种免标记的生物细胞活性实时检测方法。该方法依据不同浓度、生理、病理状态下细胞组织的电学特性差异来判断细胞是否具有活性，以协助医师在临床手术中快速精准定位患者烫伤组织并实现有效切除。方法 使用具有活性的斑马鱼胚胎干细胞来模拟人体烫伤组织，采用生物阻抗谱技术来鉴别细胞组织的生理状态。结果 在不同状态下，细胞的阻抗幅值变化有显著的差异，可以从中发现同等浓度下活性细胞的阻抗幅值比死亡细胞平均高出17.25%，活性细胞发生弛豫频率的时间也比死亡细胞早25%。结论 实验数据表明，生物阻抗谱法能有效区分胚胎干细胞的两类生理状态；从聚类区域中可以看出，BIS检测法具有明显的细胞活性及浓度区分能力，理论上能够快速地协助医师完成对患者烫伤组织检测。

Biological Cell Activity Label-free Assay Based on Bioelectrical Impedance Spectroscopy

Objective Based on bioelectrical impedance spectroscopy (BIS), we propose a label-free method for real-time detection of biological cell activity. The method determines whether the cells are active or not based on the differences in electrical properties of the cells at different concentrations, physiological and pathological states. It is used to assist physicians to quickly and precisely locate the scalded tissues in patients and achieve effective excision during clinical surgery.Methods Active zebrafish embryonic stem cells were used to simulate human scalded tissues, and bioimpedance spectroscopy was used to identify the physiological state of the cellular tissues.Results There were significant differences in the impedance amplitude changes of the cells in different states, from which it could be found that the impedance amplitude of the active cells at the same concentration was on average 17.25% higher than that of the dead cells, and the relaxation frequency of the active cells occurred 25% earlier than that of the dead cells.Conclusion The experimental data showed that the bioimpedance spectroscopy method can effectively distinguish two types of physiological states of embryonic cells; according to the clustering region, it can be seen that the BIS assay has obvious ability to distinguish cell activity and concentration, which can quickly assist physicians to complete the detection of scalded tissues of patients theoretically.