Inhibition of nitric oxide production by natural oxyprenylated coumarins and alkaloids in RAW 264.7 cells |
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| Affiliation: | 2. Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, I-87036 Rende (CS), Italy;1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People’s Republic of China;2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peaking Union Medical College, Beijing 100050, People’s Republic of China;1. Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki 852-8521, Japan;2. Department of Chemistry, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan;3. Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan;4. Department of Life Science, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan;5. Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China;1. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China;2. Xiangya Hospital of Central South University, Changsha 410008, PR China;3. The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, PR China |
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| Abstract: | A series of naturally occurring 3,3-dimethylallyloxy- and geranyloxycoumarins and alkaloids were chemically synthesized and tested as anti-inflammatory agents for their inhibitory effects on nitric oxide production in LPS-stimulated RAW 264.7 cells. Results indicated that the alkaloid of fungal origin 3-methylbut-2-enyl-4-methoxy-8-[(3-methylbut-2-enyl)oxy]quinoline-2-carboxylate, commonly known as Ppc-1, and coumarins having an unsubstituted 2-benzopyrone ring exhibited the highest activity with IC50 values from 23 to 34 μM without having poor or not detectable cytotoxicity. Indomethacine and L-NAME used as reference drugs provided by far less activities. |
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| Keywords: | Anti-inflammatory activity Coumarins Nitric oxide Oxyprenylated secondary metabolites RAW 264.7 cells |
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