Triterpenoid saponins from Clematis chinensis and their inhibitory activities on NO production |
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| Affiliation: | 1. College of Pharmacy and Biological Engineering, Chengdu University, Chengdu 610106, China;2. College of Medicine, Chengdu University, Chengdu 610106, China;3. Department of Emergency, West China Hospital, Sichuan University, Chengdu 610041, China;1. Pharmacognosy Department, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia;2. Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt;3. Chemistry Department, Science College, Princess Noura bint Abdul Rahman University, Riyadh 11671, Saudi Arabia;4. Pharmacognosy Department, College of Pharmacy, Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia;1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Pr. 100-let Vladivostoku 159, 690022, Vladivostok, Russian Federation;2. Far Eastern Federal University, 8 Sukhanova St., 690090, Vladivostok, Russian Federation;3. Botanical Garden-Institute Far Eastern Branch of the Russian Academy of Sciences, Makovskii Str. 142, 690024, Vladivostok, Russian Federation;4. Laboratory of Experimental Oncology, Department of Oncology, Hematology and Bone Marrow Transplantation with Section Pneumology, Hubertus Wald-Tumorzentrum, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;1. School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China;2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;1. University of South Florida, États-Unis;2. École de psychologues praticiens, 23, rue du Montparnasse, 75006 Paris, France |
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| Abstract: | Four new triterpenoid saponins, clematochinenoside H–K (1–4), and five known structures (5–9), were isolated from the roots and rhizomes of Clematis chinensis. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. All isolates were evaluated for inhibitory effects against nitric oxide (NO) production in LPS-induced RAW 246.7 macrophages. Monodesmosidic saponins (1–3, 5, and 6) with a free carboxylic acid function at C-28 exhibited potent inhibitory activities with IC50 values in the range of 12.9–32.3 μM, where as bisdesmosidic saponin (4, and 7–9) showed modest inhibitory effects with the inhibition ratios (%) from 39.9 to 59.0 at 50 μM. In addition, the hydroxyl group at C-21 showed negative effect on the NO production inhibitory activity. |
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| Keywords: | Triterpenoid saponins Anti-inflammatory activity Nitric oxide production |
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