Investigation of chemical constituents from Spiraea prunifolia var. simpliciflora and their biological activities |
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| Institution: | 1. Central Siberian Botanical Garden of Siberian Branch of Russian Academy of Sciences (CSBG SB RAS), 101 Zolotodolinskaya Str., Novosibirsk 630090, Russia;2. Tomsk State University, 36 Lenin Ave., Tomsk 634050, Russia;4. Amur Branch of Botanical Garden-Institute of the Far Eastern Branch Russian Academy of Science (AB BGI FEB RAS), Ignatievskоe Road, 2km, Blagoveshchensk 675000, Russia |
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| Abstract: | Quantum mechanics (QM)-based calculations for elucidating full structures of natural compounds are growing in importance and reliability. Two new phenolic glycosides (1 and 2) and 11 known compounds were isolated from the twigs of Spiraea prunifolia var. simpliciflora. The chemical structures of the new compounds (1 and 2) were initially established through different NMR techniques (1H and 13C NMR, COSY, HSQC, and HMBC), HRMS data analysis, and chemical hydrolysis. These structure assignments were further verified by QM-based NMR chemical shift calculations. All of the purified compounds (1–13) were evaluated for their cytotoxicity against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and BT549). Those phytochemicals were also evaluated for both anti-inflammatory activity through the measurement of nitric oxide (NO) production levels in lipopolysaccharide (LPS)-stimulated murine microglia BV-2 cell lines and neuroprotective effects via induction of nerve growth factor (NGF) in C6 glioma cells. |
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| Keywords: | Phenolic glycosides GIAO NMR chemical shift calculations Cytotoxicity Anti-inflammation Neuroprotection |
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