Phosphorylation and nucleotidylation of proteins in Rhodocyclus gelatinosus |
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Authors: | Beate Averhoff Garabed Antranikian Gerhard Gottschalk |
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Institution: | Institut fur Mikrobiologie der Universität Göttingen, Grisebachstrasse 8, D - 3400 Göttingen, F.R.G. |
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Abstract: | Abstract Cells of Rhodocyclus gelatinosus were radioactively labeled by addition of 32P]orthophosphate, 14C]inosine or 14C]orotic acid during anaerobic growth on citrate in the light. Protein analysis by two-dimensional gel electrophoresis and autoradiography of the gels revealed the presence of several radioactively labeled protein species in this organism. The molecular mass and the isoelectric point of all these proteins were determined. Treatment of the 32P-labeled protein fractions with acid and alkaline phosphatase clearly showed that at least 8 protein species were modified by phosphorylation. The experiments conducted with the 14C-labeled precursors of purines and pyrimidines indicated the presence of 4 protein species which were modified by a compound containing a purine and phosphate, and a single protein simultaneously being labeled with pyrimidine and phosphate. |
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Keywords: | Protein phosphorylation protein nucleotidylation two-dimensional analysis alkaline phosphatase acid phosphatase Rhodocyclus gelatinosus |
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