Differential molecular responses to abscisic acid and osmotic stress in viviparous maize embryos |
| |
Authors: | William M. Butler Andrew C. Cuming |
| |
Affiliation: | (1) Department of Genetics, Leeds University, LS2 9JT Leeds, UK |
| |
Abstract: | Substantial quantities of mRNA encoding the abundant Em polypeptide accumulate, in planta, in developing embryos of maize (Zea mays L.). By contrast, accumulation of Em mRNA is only barely detectable in embryos with the vp-5/vp-5 genotype [an abscisic acid (ABA)-deficient viviparous phenotype]. Em mRNA is not detectable within viviparous embryos of the vp-1/vp-1 genotype that are non-responsive to ABA. Culture of immature wild-type and vp-5/vp-5 embryos in the presence of exogenous ABA or of an osmotically active agent prevents precocious germination and results in expression of the Em genes. When vp-1/vp-1 embryos are cultured under similar conditions, only the application of osmotic stress prevents precocious germination. However, Em mRNA does not accumulate either in ABA-treated or stressed, arrested embryos, indicating a requirement for ABA perception through a VP-1-mediated mechanism for Em gene expression. Nevertheless, vp-1/vp-1 embryos do show both ABA and stress responses at the molecular level. Treatment with ABA causes the accumulation of mRNA encoding a polypeptide of approx. 30 kDa, whilst osmotic stress induces the accumulation both of a 30-kDa polypeptide and a set of approx. 20-kDa polypeptides. This indicates the existence of discrete, parallel ABA and stress response pathways in developing maize embryos.Abbreviations ABA abscisic acid - cDNA copy-DNA - DAP days after pollination - kDa kilodaltons - MS Murashige and Skoog medium - LEA late embryogenesis abundant - NEpHGE non-equilibrium pH gradient gel electrophoresis - SDS-PAGE sodium dodecyl sulphate-polyacrylamide gel electrophoresis |
| |
Keywords: | Abscisic acid Cereal embryogenesis /content/l277495t8161w75k/xxlarge8220.gif" alt=" ldquo" align=" MIDDLE" BORDER=" 0" >Em /content/l277495t8161w75k/xxlarge8221.gif" alt=" rdquo" align=" MIDDLE" BORDER=" 0" > genes Osmotic stress |
本文献已被 SpringerLink 等数据库收录! |
|