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| 蒙古牛BMPR-IB基因部分cDNA的克隆和序列分析 | |
| 引用本文： | 董文杰,王峰,刘永斌,田春英,刘美霞,荣威恒. 蒙古牛BMPR-IB基因部分cDNA的克隆和序列分析[J]. 生物技术, 2007, 17(4): 3-6 |
| 作者姓名： | 董文杰 王峰 刘永斌 田春英 刘美霞 荣威恒 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 2. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农牧业科学院,内蒙古,呼和浩特,010030 3. 内蒙古农牧业科学院,内蒙古,呼和浩特,010030 4. 内蒙古医学院第二附属医院,内蒙古,呼和浩特,010010 |
| 摘 要： | BMPR-IB基因主要在哺乳动物卵巢中表达,对卵泡的发育和分化起重要作用。该研究从影响卵巢生长发育和调控的BMPR-IB基因出发,以牛卵巢的RNA为模板,按照不同物种BMPR-IB基因的相似性设计特异引物,运用RT-PCR技术扩增并获得了特异片段,该片段经PCR、酶切和测序验证,证实所克隆序列为牛BMPR-IB序列,包含有953bp组成的部分cDNA序列,同源性分析结果表明,牛BMPR-IB基因序列与绵羊、山羊、人、猪、小鼠的BMPR-IB基因分别为98%、97%、92%、93%、88%的同源性。这为克隆其他物种的BMPR-IB基因提供了依据,同时牛骨形态发生蛋白的测序为更好地理解牛的生殖机理提供帮助。 |
| 关 键 词： | 牛 克隆 序列分析 |
| 文章编号： | 1004-311X（2007）04-0003-04 |
| 收稿时间： | 2007-01-25 |
| 修稿时间： | 2007-03-26 |
Sequence Analysis and cDNA Cloning of Mongolian Cow Bone Morphogenetic Protein Receptor-IB Gene |
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| DONG Wen-jie,WANG Feng,LIU Yong-bin,TIAN Chun-ying,LIU Mei-xia,RONG Wei-heng. Sequence Analysis and cDNA Cloning of Mongolian Cow Bone Morphogenetic Protein Receptor-IB Gene[J]. Biotechnology, 2007, 17(4): 3-6 | |
| Authors: | DONG Wen-jie WANG Feng LIU Yong-bin TIAN Chun-ying LIU Mei-xia RONG Wei-heng |
| Affiliation: | 1. College of Animals Science anti Medicine, Inner Mongolia Argiculture University,Huhhot 010018,China;2.Inner Mongolia Academy of Animal Sciences ,Huhhot 010030,China;2.The Second Accessory Hospital ,Academy of Inner Mongolia Medicine Sciene,Huhhot 010010,China |
| Abstract: | BMPR-IB has a important role to the development and differentiation of follicular and is specialized expressed in mammalian ovary.The study obtained the partial cDNA sequence of Mongolian cow BMPR-IB gene using RT-PCR methods,and was identitied by PCR and sequence,The open reading frame of BMPR-IB gene contains 953bp nucleotides,The gene and had the highest similarity with sheep,goat,human,pig and rat with percent identities being 98%,97%,92%,93% and 88%.This provided foundation for cloning the other animals BMP gene,At the same time,sequence of cow BMPR-IB gene offer help for understanding cow reproduction mechanism. |
| Keywords: | BMPR-IB RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |