IL-2-dependent in vivo and in vitro tyrosine phosphorylation of IL-2 receptor gamma chain. |
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Authors: | H Asao S Kumaki T Takeshita M Nakamura K Sugamura |
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Institution: | Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan. |
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Abstract: | We previously reported a molecule, p64, which was tentatively named the gamma chain, coprecipitable with the beta chain of human interleukin-2 receptor (IL-2R). The present study demonstrated that the gamma chain, as well as the beta chain expressed on IL-2-responsive cells, is phosphorylated on tyrosine residues in an IL-2-dependent manner in vivo and in vitro. The in vivo tyrosine phosphorylation of both chains was similarly induced within 1 min after IL-2 stimulation, and their in vitro tyrosine phosphorylation with the anti-IL-2R beta antibody-directed immunocomplex was also increased by treatment of cells with IL-2. These results suggest that a tyrosine kinase is associated with the beta gamma subunit complex, of which activation by IL-2 may result in transduction of intracellular signals. |
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