Detrimental effect of hyperosmolality on insulin-stimulated glucose metabolism in adipose and muscle tissue in vitro |
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| Authors: | M Komjati G Kastner W Waldh?usl P Bratusch-Marrain |
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| Affiliation: | 1. Tianjin Key Laboratory of Modern Chinese Medicine Theory of Innovation and Application, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2. Research Center of Experimental Acupuncture Science, School of Acupuncture-Moxibustion and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3. Ministry of Education, and State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, China;4. School of Intergrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;1. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion), Tianjin, China;2. Department of integrated Chinese and Western medicine, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China;1. USDA, Agriculture Research Service, U.S. Meat Animal Research Center, Clay Center, NE 68933 0166, USA;2. Division of Animal Sciences, University of Missouri, Columbia, MO 65211 5300, USA;1. College of Food Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou, Guangdong 510640, China;2. College of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Dongsha Street 24, Guangzhou, Guangdong 510225, China;3. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Wushan Road 381, Guangzhou, Guangdong 510640, China |
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| Abstract: | To better understand impairment of glucose utilization in diabetics during a hyperosmolal state, in vitro models were established to evaluate the interdependence of hyperosmolality on basal as well as insulin-dependent glucose uptake by rat epididymal fat pads and diaphragms. Using the epididymal fat pad it was shown that NaCl and urea induced hyperosmolality of 400 and 500 mOsm/kg diminished insulin-stimulated glucose uptake by 35 and 90%, as well as 29 and 68%, respectively. Using rat diaphragm as target tissue for insulin action instead a transient rise in basal (non-insulin-dependent) glucose uptake was seen at 400 but not at 500 mOsm/kg. Associated impairment of insulin-dependent glucose uptake was 30 and 79%, respectively. These in vitro data support our previous clinical contention that a hyperosmolal state, which corresponds to a loss of fluid in excess of solutes, is able to impair basal glucose utilization as well as hormone action on glucose metabolism. |
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