Factors affecting growth of cell suspension cultures of hypericum perforatum L. (St. John's wort) and production of hypericin |
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Authors: | Harsh?Pal?Bais Travis?S?Walker Janeen?J?McGrew Email author" target="_blank">Jorge?M?VivancoEmail author |
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Institution: | (1) Department of Horticulture and Landscape Architecture, Colorado State University, 80523-1173 Fort Collins, CO |
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Abstract: | Summary Use of Hypericum perforatum L., commonly known as St. John's wort, has increased recently due to the pharmaceutical potential
of hypericin, found in its leaves. Hypericin has been reported to effect a natural treatment for mild and moderate depression
by increasing the concentration of neurotransmitters in the central nervous system. We have developed a novel cell culture
system for in vitro growth and production of this species, suggesting a possible technology for large-scale production of
hypericin. Leaf explants grown in Murashige and Skoog salts supplemented with 2,4-dichlorophenoxyacetic acid (0.90 μM) and
kinetin (0.11 μM) gave maximum percentage callus formation compared to other medium treatments evaluated. Hypericin localization
in cell phase and leaves was found to vary, with cell phase accumulating hypericin in special organelles and leaves accumulating
it in vacuoles. Light and dark conditions, with cell aggregate size, played important roles in growth and hypericin production
in cell suspension cultures. |
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Keywords: | Hypericum perforatum St John's wort callus cultures suspension cultures hypericin |
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