GABA modulation of cholinergic transmission in rat oviduct |
| |
Authors: | I Fernández LM Orensanz ML de Ceballos |
| |
Institution: | 1. Departamento de Investigación, Centro Ramón y Cajal, Madrid 34, Spain;2. Departamento de Bioquímica, C.U.I. Arcos de Jalón, Madrid, Spain;3. Departamento de Farmacología, Instituto Ramón y Cajal, C.S.I.C., Velazquez 144, Madrid 6, Spain |
| |
Abstract: | The effects of electrical stimulation, γ-aminobutyric acid (GABA), acetylcholine (ACh), norepinephrine (NE), 5-hydroxytryptamine (5-HT), GABA agonists and bicuculline were studied on spontaneous movements of isolated rat oviduct. The tissue did not respond to electrical stimulation or to GABA, NE and 5-HT when added to the incubation medium. ACh produced contractions related to its concentration which were maximal at the diestrous-1 phase when GABA caused a 20% rise in the ACh contraction. This effect was mimicked by GABA agonists whereas it was suppressed by bicuculline. β-Estradiol benzoate (EB) increased ACh contractions in diestrous-1 and in the late proestrous phases. GABA did not modify the EB effect. Progesterone did not modify ACh contractions in any of the studied phases. These findings suggest a possible modulatory role for GABA on ACh responses in the isolated rat oviduct. |
| |
Keywords: | To whom correspondence should be addressed |
本文献已被 ScienceDirect 等数据库收录! |
|