Analysis of Bacillus megaterium lipolytic system and cloning of LipA,a novel subfamily I.4 bacterial lipase |
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| Authors: | Ruiz Cristian Blanco Ana Pastor F I Javier Diaz Pilar |
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| Affiliation: | Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain. |
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| Abstract: | The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases. |
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| Keywords: | Bacillus Bacillus megaterium Lipase Carboxylesterase |
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