Arterial-venous endothelial cell fate is related to vascular endothelial growth factor and Notch status during human bone mesenchymal stem cell differentiation |
| |
Authors: | Zhang Gang Zhou Jianye Fan Quanxin Zheng Zhe Zhang Fusheng Liu Xuebin Hu Shengshou |
| |
Affiliation: | Department of Cardiac Surgery, Shandong Provincial Hospital, Shandong University, Jingwu Road 324, Jinan 250012, PR China. |
| |
Abstract: | Human bone mesenchymal stem cells (hMSCs) can differentiate into endothelial cells (ECs), so we aimed to investigate whether hMSCs could also differentiate into a specific arterial or venous ECs. hMSCs were induced to differentiate into ECs using vascular endothelial growth factor (VEGF). Low VEGF concentration (50ng/ml) upregulated the venous marker gene EphB4, however high concentration (100ng/ml) upregulated the arterial marker genes ephrinB2, Dll4 and Notch4, and downregulated the venous marker genes EphB4 and COUP-TFll. This VEGF dose-dependent induction was largely blocked by inhibition of the Notch pathway in hMSCs treated with gamma-secretase inhibitor. Therefore, differentiation of hMSCs into arterial- or venous-specific ECs depends on VEGF and is regulated by the Notch pathway. |
| |
Keywords: | Endothelial cell Mesenchymal stem cell Arteries Veins |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|