Improved technique for the reversion of Histoplasma capsulatum in tissue culture

Summary Reversion ofHistoplasma capsulatum mycelia to the parasitic yeast phase was accomplished in stationary cultured HeLa cells. Fresh guinea pig serum in either Hely or Medium 199 gave excellent conversion. Horse, human, calf, and chicken serum permitted conversion, but to a lesser extent as measured by subsequent growth on blood agar. No apparent differences were found with respect to conversion between 2, 4, and 8-week old mycelial inoculum.Work reported in this paper was supported in part by Grant 2 T1 AI 123 of the National Institutes of Health, U.S. Public Health Service.