Organisation of nodulation and nitrogen fixation genes on a Rhizobium trifolii symbiotic plasmid |
| |
| Authors: | D. Barry Scott Chris B. Court Clive W. Ronson Kieran F. Scott John M. Watson Peter R. Schofield John Shine |
| |
| Affiliation: | (1) Applied Biochemistry Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand;(2) Grasslands Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand;(3) Centre for Recombinant DNA Research, Research School of Biological Sciences, Australian National University, PO Box 475, 2601 Canberra City, Australia |
| |
| Abstract: | A Rhizobium trifolii symbiotic plasmid specific gene library was constructed and the physical organisation of regions homologous to nifHDK, nifA and nod genes was determined. These symbiotic gene regions were localised to u 25 kb region on the sym-plasmid, pPN1. In addition four copies of a reiterated sequence were identified on this plasmid, with one copy adjacent to nifH. No rearrangement of these reiterated sequences was observed between R. trifolii bacterial and bacteroid DNA. Analysis of a deletion derivative of pPN1 showed that these sequences were spread over a 110 kb region to the left of nifA. |
| |
| Keywords: | Rhizobium trifolii Symbiosis Nodulation Nitrogen fixation Symbiotic genes Reiterated sequences Plasmid |
| 本文献已被 SpringerLink 等数据库收录！ |
|
