| Abstract: | The bioassay-guided fractionation of H. oblongifolium has led to the isolation of potent urease inhibitors 1–3. The structures were elucidated by NMR and mass spectroscopic techniques. Compound 2 showed a potent enzyme inhibition activity (IC50 20.96?±?0.93), which is comparatively higher than that for the standard thiourea (IC50 21.01?±?0.51 μM). Compounds 1 and 3 also showed a significant activity, with IC50 37.95?±?1.93 and 138.43?±?1.23 μM, respectively. The sub crude fractions (F1, F2, F3, and F4) were tested in vitro for their urease inhibition activity. Fractions F2 and F4 showed significant activity with IC50 140.37?±?1.93 and 167.43?±?3.03 μM, respectively. |
