双孢蘑菇Septin基因Ab.Cdc3的克隆与分析

具有 GTPase 活性的 Septin蛋白广泛存在于除植物外的所有真核生物中,其功能多样。采用tBlastn将灰盖鬼伞Coprinus cinereus中与菌柄伸长相关的Septin蛋白Cc.Cdc3与双孢蘑菇基因组数据进行比对,在双孢蘑菇基因组中找到Cc.Cdc3同源蛋白编码基因Ab.Cdc3。生物信息学分析结果表明,Ab.Cdc3蛋白序列具有保守GTPase结构功能域。通过荧光定量PCR（quantitative real-time PCR,qRT-PCR）方法分析Ab.Cdc3在不同生长阶段的表达模式,结果显示该基因在子实体菌柄中表达量较菌丝、原基和菌盖中的表达量高;采后不同保藏时间样品Ab.Cdc3基因表达分析结果显示,在采后0h表达量显著高于采后12h和48h;这些结果为进一步研究该基因在双孢蘑菇生长发育中的功能提供参考。

Cloning and expression analyses of the septin gene Ab.Cdc3 in Agaricus bisporus

Septins with GTPase activity are widely found in eukaryotic organisms except for plants, and play multiple roles. Based on the local tBlastn analysis, Coprinus cinereus septin gene Cc.Cdc3 was contrasted with the genome of Agaricus bisporus, and a homologue gene in A. bisporus was identified and termed Ab.Cdc3. Alignment of Ab.Cdc3 with homologous septins revealed the presence of the conserved motifs within GTP-binding domain. Quantitative real-time PCR revealed that the expression of Ab.Cdc3 was higher in stipe than that in mycelium, primordium and caps during different developmental stages. Subsequent analysis showed that the expression of this gene in the post-harvest fruiting body was significantly higher at 0h than that at 12h and 48h. These results provide valuable reference for further investigating the function of Ab.Cdc3 during fruiting body development in A. bisporus.