Nucleotide sequence of the aliphatic amidase regulator gene (amiR) of Pseudomonas aeruginosa |
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| Authors: | N Lowe P M Rice R E Drew |
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| Affiliation: | Department of Biochemistry, University College London, England. |
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| Abstract: | The nucleotide sequence of a 1001 bp ClaI/XhoI DNA fragment encoding the amidase regulator gene (amiR) from Pseudomonas aeruginosa has been determined. The sequence derives from strain PAC433, a constitutive high expressing amidase mutant, and contains two overlapping open reading frames. Analysis of the sequence has identified one of the reading frames as amiR. The gene encodes a 196 amino acid polypeptide which shows a strong bias towards codons with G or C in the third position. The amiR gene shows no sequence homology with other bacterial regulator proteins. |
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