A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous fungi |
| |
| Authors: | Kato M Hayashi K Kobayashi T Tsukagoshi N |
| |
| Affiliation: | Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan. kato@agr.nagoya-u.ac.jp |
| |
| Abstract: | A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
