A region of primer binding variation at the D6S265 locus associated with HLA-A25 and HLA-A26 antigens |
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Authors: | Wendy Pyper Michael Burt Lawrie Powell Sonja Webb Lesley Adès June Halliday Elizabeth Jazwinska |
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Institution: | (1) Queensland Institute of Medical Research and Department of Medicine, University of Queensland, 4029 Brisbane, Australia;(2) Department of Medical Genetics, Adelaide Childrens Hospital, 5006 North Adelaide, S.A., Australia |
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Abstract: | D6S265 is a polymorphic dinucleotide repeat, mapped within 70 kb centromeric of HLA-A, on chromosome 6p21.3. While genotyping families for genetic linkage analysis, allele non-amplification resulting in apparent non-Mendelian inheritance was observed at the D6S265 locus in 15 individuals, on chromosomes carrying the HLA-A25 and HLA-A26 antigens. The D6S265 locus was sequenced in a variant individual homozygous for allele non-amplification, and in a non-HLA-A25/-A26 individual, homozygous for D6S265 allele 1. Five base changes were identified in the reverse primer binding region of the variant individual, effectively preventing annealing of the 3 primer to the template. |
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