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Influence of nutritional conditions on exopolysaccharide production by submerged cultivation of the medicinal fungus Shiraia bambusicola
Authors:Hailong Yang  Guoqing He
Affiliation:(1) College of Biosystem Engineering and Food Science, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, People’s Republic of China;(2) School of Life and Environmental Sciences, Wenzhou University, 276 Xueyuan Road, Wenzhou, 325027, People’s Republic of China
Abstract:Maltose and yeast extract were the most favourable carbon and nitrogen sources for exopolysaccharide production by submerged culture of Shiraia bambusicola WZ-003, and initial maltose and yeast extract concentrations were at 30 and 3 g l−1, respectively. Plant oils could increase the mycelial growth and exopolysaccharide production in tested concentration. K+ and Mg2+ could enhance the mycelial growth and exopolysaccharide biosynthesis. The optimal cultivation temperature and initial pH were found to be 26°C and 6.0, respectively. Exopolysaccharide concentration reached 0.53 g l−1 in 15-l fermenter under optimal nutritional conditions.
Keywords:Exopolysaccharide  Medicinal fungus  Production   Shiraia bambusicola   Submerged culture
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