Effects of nonuniform column packing in analytical gel chromatography. II. Associating solutes |
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| Authors: | J K Zimmerman G K Ackers |
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| Affiliation: | Department of Biochemistry, Clemson University, Clemson, South Carolina 29631 USA;Department of Biochemistry, University of Virginia, Charlottesville, Virginia 22901 USA |
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| Abstract: | Effects of nonuniform column packing on boundary profiles for selfassociating systems have been investigated by computer simulation. Migration rate of each of the interconverting solute species changes along the column as a result of nonuniform packing, and the difference in velocity of monomer and n-mer is not constant as the sample moves down the column. A greater amount of overall axial dispersion results, as compared to the constant-column case. Procedures developed in this study can be applied to any experimentally measured column nonuniformity. |
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| Keywords: | To whom inquiries should be addressed. |
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