Lentiviral reporter constructs for fluorescence tracking of the temporospatial pattern of Smad3 signaling |
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Authors: | Stuelten Christina H Kamaraju Anil K Wakefield Lalage M Roberts Anita B |
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Affiliation: | Cell and Cancer Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4254, USA. chrisstu@mail.nih.gov |
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Abstract: | Canonical TGF-beta is involved in cell differentiation, tissue maintenance, and wound healing, but also plays a central role in the pathogenesis of diseases such as cancer Here we describe a lentivirus-based reporter vector system expressing green fluorescent protein (GFP) or red fluorescent protein (RFP) under the control of a Smad3-responsive element (CAGA)12 that allows observation of the temporospatial pattern of endogeneous Smad3-mediated signaling on a cellular level. Use of this method will be valuable to identify cells with active Smad3 signaling and investigate the role of endogenous Smad3 signaling in complex systems such as co-cultures in vitro, or in tumors during tumor cell invasion and metastasis in vivo. |
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