Adrenal oxidative enzymes in ox and sheep. The histochemical demonstration of some pathways involved in chromaffin granule synthesis

Summary Catechol amine secretion is achieved by exocytosis. In this, ATP and protein are also lost from the chromaffin cells. Histochemically various specific coenzyme linked dehydrogenases associated with ATP production have been demonstrated in the adrenals of ox and sheep. These included cytochrome oxidase, DPN and TPN diaphorases, isocitric dehydrogenase, malate dehydrogenase, glutamate dehydrogenase, succinic dehydrogenase, lactic dehydrogenase, alcohol dehydrogenase, -glycerophosphate dehydrogenase and -hydroxybutyrate dehydrogenase. Enzymes of the pentose shunt were found histochemically and biochemically. The RNA content of the adrenal medulla and cortex was also investigated.