Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis |
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Authors: | Choun-Sea Lin Yi-Hwa Lai Chih-Wen Sun Nien-Tzu Liu Hsing-Shen Tsay Wei-Chin Chang Jeremy J W Chen |
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Institution: | (1) Department of Biotechnology, China Institute of Technology, Taipei, Taiwan, Republic of China;(2) Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan, Republic of China;(3) Department of Life Sciences, National Taiwan Normal University, Taipei, Taiwan, Republic of China;(4) Graduate Institute of Biotechnology, ChaoYang University of Technology, Wufong, Taichung, Taiwan, Republic of China;(5) Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China;(6) Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan, Republic of China |
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Abstract: | To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant. Ten expressed sequence tags (ESTs) were found to be differentially expressed; these were isolated and sequenced. RT-PCR analysis of these ESTs supported the results of the microarray analysis. Six ESTs that were nucleus-encoded exhibited differential expression patterns in the green wild-type bamboo relative to the albino mutant. These genes (exception being the Rubisco small subunit) were non-photosynthesis-related genes. The development of a specific SSH cDNA library in which most of the chloroplast-encoded or photosynthesis-related genes had been subtracted proved to be useful for studying the function of non-photosynthesis-related genes in the albino bamboo mutants with aberrant chloroplast genome. The combined use of this SSH library with microarray analysis will provide a powerful analytical tool for future studies of the bamboo genome. |
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