Effect of ionic liquid on the kinetics of peroxidase catalysis |
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Authors: | Lee Yoon-Mi Kwon O-Yul Yoo Ik-Keun Ryu Keun-Garp |
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Institution: | Department of Chemical Engineering and Bioengineering, University of Ulsan, Ulsan 680-749, Korea. |
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Abstract: | The effect of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM]BF4]), on the horseradish peroxidase (HRP)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide (H2O2) was investigated. HRP maintains its high activity in the aqueous mixtures containing various concentrations of the ionic liquid and even in 90% (v/v) ionic liquid. In order to minimize the effect of solution viscosity on the kinetic constants of HRP catalysis, the enzymatic reactions in the subsequent kinetic study were performed in water-ionic liquid mixtures containing 25% (v/v) ionic liquid at maximum. As the concentration of BMIM]BF4] increased for the oxidation of guaiacol by HRP, the K(m) value increased with a slight decrease in the k(cat) value: The K(m) value increased from 2.8 mM in 100% (v/v) water to 22.5 mM in 25% (v/v) ionic liquid, indicating that ionic liquid significantly weakens the binding affinity of guaiacol to HRP. |
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