A method for the isolation of phage mutants altered in their response to lysogenic induction |
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Authors: | David W. Mount |
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Affiliation: | (1) Department of Microbiology, College of Medicine, University of Arizona, 85724 Tucson, Arizona, U.S.A. |
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Abstract: | Summary Phage cl+ gives clear plaques whereas phage cIind- gives turbid plaques on a lawn of a mutant strain of E. coli K12. This strain, called STS, carries mutation spr in a tif sfi genetic background. I hypothesize that upon temperate phage infection, STS bacteria spontaneously inactivate phage repressor by the same mechanism involved in normal lysogenic induction which results in obligatory lytic growth of +. The use of the STS mutant facilitates the isolation and genetic analysis of phage mutants with an abnormal response to lysogenic induction. |
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