Ultrastructural and autoradiographic observations of hamster cheek pouch epithelium grown in vitro |
| |
Authors: | Baldev B Singh George S Schuster Virginia A Merchant Virginia J Michelich |
| |
Institution: | (1) Department of Oral Pathology, Medical College of Georgia, 30901 Augusta, Georgia;(2) Department of Oral Microbiology, Medical College of Georgia, 30901 Augusta, Georgia;(3) Department of Cell and Molecular Biology, Medical College of Georgia, 30901 Augusta, Georgia |
| |
Abstract: | Summary Epithelial outgrowths from hamster cheek pouch explants were cultured for varying peroids of time up to 22 days. Growth of
the epithelial sheets was monitored, employing colcemid for demonstrating mitotic activity and tritiated thymidine for DNA
synthesis. Mitoses and thymidine uptake were observed among epithelial outgrowths at a considerable distance from the original
explant. The epithelial nature of the growing cell sheets was confirmed, employing electron microscopic techniques. The cells
exhibited the presence of tonofilaments, desmosomes, ribosomes, Golgi, mitochondria, and rough endoplasmic reticulum. The
cultured explants were treated with cyclic nucleotides in order to investigate their modulatory effects on epithelial cell
differentiation. Dibutyryl cAMP induced marked mitotic inhibition (46.3%) in our assay, which was increased to 57% with the
addition of theophylline. Dibutyryl cGMP showed only a mild (5%) stimulatory effect on mitotic activity. Dibutyryl cAMP enhanced
keratinization in the epithelial cell out-growths with the biogenesis of keratohyalin granules, whereas dibutyryl cGMP did
not produce any observable alterations. |
| |
Keywords: | cyclic nucleotides epithelial cultures ultrastructure hamster cheek pouch |
本文献已被 SpringerLink 等数据库收录! |
|