Degradation of glucagon receptors by dispase treatment of isolated intact rat hepatocytes |
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| Authors: | Vera Pingoud Theodor Haas Ivar Trautschold |
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| Affiliation: | Zentrum Biochemie, Medizinische Hochschule Hannover, Konstanty-Gutschow-Str. 8, D-3000 Hannover 61 F.R.G. |
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| Abstract: | Collagenase-isolated rat hepatocytes were treated with dispase II, a neutral proteolytic enzyme which is often used for the disintegration of neonatal cells. The treatment of hepatocytes with dispase II caused a significant reduction of glucagon binding to the intact cells. The deleterious effect of this enzyme on the specific glucagon binding sites is accompanied by a reduction of the maximum intracellular cyclic AMP production. |
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| Keywords: | Glucagon Hormone receptor Protein degradation Dispase (Rat hepatocyte) |
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