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Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation |
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| Authors: | Annette Droste Giancarlo Pasquali Maria Helena Bodanese-Zanettini |
| Affiliation: | 1. Centro de Ciências da Saúde, Universidade do Vale do Rio dos Sinos, Caixa Postal 275, 93022-000, S?o Leopoldo, RS, Brazil 2. Centro de Biotecnologia do Estado do Rio Grande do Sul e Departamento de Biotecnologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, 91501-970, Porto Alegre, RS, Brazil 3. Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-970, Porto Alegre, RS, Brazil |
| Abstract: | Described here is a new method for soybean transformation, based on microwounding of embryonic clumps by particle bombardment prior to inoculation with anAgrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation ofGlycine max. |
| Keywords: | Agrobacterium Glycine max particle bombardment somatic embryogenesis transformation |
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