Species differences in the coexistence of 5-hydroxytryptamine and substance P in presynaptic boutons in the cervical ventral horn |
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Authors: | H Sakamoto S Atsumi |
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Institution: | (1) Department of Anatomy, Yamanashi Medical College, Tamaho, 409-38 Yamanashi, Japan |
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Abstract: | Summary Substance P (SP)-and 5-hydroxytryptamine (5-HT)-containing presynaptic boutons in the cervical ventral horn were studied in chicken, hamster, rat and monkey spinal cords, using PAP and protein A-gold double-labeling techniques in conjunction with monoclonal antibodies. In the chicken, the PAP method demonstrated that SP-immunoreactive boutons contained large spherical dense-cored vesicles (DCVs) whereas 5-HT-immunoreactive boutons displayed both elongated and spherical DCVs. Using the protein A-gold double-labeling technique, 10-nm gold particles for SP were localized over the spherical DCV-containing boutons whereas 15-nm gold particles for 5-HT were localized on elongated DCV-containing boutons. On the other hand, in the other species investigated, both SP-and 5-HT-immunoreactive boutons had similar morphological features as shown by the PAP method; both contained elongated and spherical DCVs. The two differentsized gold particles, each of which labeled either 5-HT or SP, were found together over DCVs in a single bouton. These results indicate that 5-HT and SP are contained in different presynaptic boutons in the chicken, although in the hamster, rat and Japanese macaque, the two neurotransmitters/modulators coexist in the same DCVs in a single bouton. Species differences have thus been demonstrated for the coexistence of 5-HT and SP in the spinal ventral horn. |
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Keywords: | Substance P 5-Hydroxytryptamine (serotonin) Spinal cord Ventral horn Immuno-electron microscopy Chicken Rat (Wistar) Golden hamster Macaca fuscata |
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