Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase |
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Authors: | Pilar Reyes Michael A Pickard Rafael Vazquez-Duhalt |
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Institution: | (1) Instituto de Biotecnologia-UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62250, Mexico;(2) Department of Biological Sciences, University of Alberta, Edmonton, Canada, T6G 2E9 |
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Abstract: | Laccase from Coriolopsis gallica UAMH8260 was immobilized on activated agarose and tested for repeated decolorization of industrial dyes. Immobilized enzyme retained 85% of the initial activity after 10 cycles, and 70% after 3 months of intermittant use in the decolorization of Reactive Blue 198 dye. Free laccase decolorized 13 of 38 industrial dyes tested but, in the presence of 1 mM 1-hydroxybenzotriazole as a free radical mediator, the enzyme decolorized 26 of the 38 dyes increasing both the range and rate of decolorization. Immobilized laccase showed a higher thermal stability at 70 °C than free enzyme but no increased resistance to organic solvents. |
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Keywords: | dye immobilized enzyme laccase mediators textile wastewater |
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