Immune Responses to HBsAg Conjugated to Protein D of Non-Typeable Haemophilus influenzae in Mice |
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Authors: | Qiudong Su Yao Yi Feng Qiu Xuexin Lu Junying Ding Zhiyuan Jia Ruiguang Tian Yan Gao Shengli Bi |
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Affiliation: | National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing, China;University of Melbourne, AUSTRALIA |
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Abstract: | BackgroundHepatitis B vaccine that contains an aluminum hydroxide adjuvant induces apoptotic death of Hepa 1–6 cells. Difficult-to-degrade chemical additives in vaccines effectively enhance vaccine immunogenicity, but also affect the host tissue. Identification of bio-molecules that are readily degraded and compatible in vivo as an adjuvant is important for vaccine research. The hapten–carrier effect suggests that stimulation of helper T (Th) cells by carrier adjuvants is feasible. Protein D (PD) of non-typeable Haemophilus influenzae covalently conjugated to some polysaccharide vaccines has been confirmed to convert T-cell independent (TI) antigens into T-cell dependent (TD) antigens, and elicit strong T-cell responses ultimately. Herein, we would substitube PD for aluminum hydroxide adjuvant in Hepatitis B vaccine.ConclusionsRecombinant truncated PD covalently conjugated to HBsAg antigen enhanced the immunogenicity of the antigen in mice simultaneously by humoral and cellular immune response, which would facilitate therapeutic hepatitis B vaccines. |
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