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Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis |
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| Authors: | Dongdong Mu Jiaojiao Lu Chang Shu Haowen Li Xingjiang Li Jing Cai |
| Affiliation: | 1. School of Food Science and Engineering, Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei University of Technology, Hefei, China;2. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, Chinad.mu@hfut.edu.cn |
| Abstract: | Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase was performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min). |
| Keywords: | Microbial transglutaminase enzymatic activity thermostability penta-site mutagenesis Escherichia coli |