Functional properties of anti-inflammatory substances from quercetin-treated Bifidobacterium adolescentis |
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Authors: | Kyuichi Kawabata Nobuyuki Baba Taiken Sakano Yoshimitsu Hamano Shu Taira Akira Tamura |
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Affiliation: | 1. Faculty of Nutrition, Kobe Gakuin University, Kobe, Japankyuichi@nutr.kobegakuin.ac.jp;3. Department of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan;4. Lactic Acid Bacteria Research Department, Food Science Research Laboratories R&5. D Division, Meiji Co., Ltd., Odawara, Japan |
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Abstract: | AbstractThe genus Bifidobacterium is well known to have beneficial health effects. We discovered that quercetin and related polyphenols enhanced the secretion of anti-inflammatory substances by Bifidobacterium adolescentis. This study investigated characteristics of the anti-inflammatory substances secreted by B. adolescentis. The culture supernatant of B. adolescentis with quercetin reduced the levels of inflammatory mediators in activated macrophages. Spontaneous quercetin degradant failed to increase anti-inflammatory activity, while the enhancement of anti-inflammatory activity by quercetin was sustained after washout of quercetin. Physicochemical treatment of the culture supernatant indicated that its bioactive substances may be heat-stable, non-phenolic, and acidic biomolecules with molecular weights less than 3 kDa. Acetate and lactate have little or no effect on nitric oxide production. Taken together, the anti-inflammatory substances secreted by B. adolescentis may be small molecules but not short chain fatty acids. In agreement with these findings, stearic acid was tentatively identified as a bioactive candidate compound. |
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Keywords: | Bifidobacterium adolescentis quercetin anti-inflammatory activity culture supernatant bioactive substances |
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