Characterisation and diversity of Indian isolates of Ralstonia solanacearum causing bacterial wilt of Capsicum annuum L. |
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| Authors: | Dinesh Singh Garima Chaudhary D. K. Yadav |
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| Affiliation: | 1. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, Indiadinesh_iari@rediffmail.com;3. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India |
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| Abstract: | Genetic diversity of 13 isolates of Ralstonia solanacearum causing bacterial wilt in hot pepper and bell pepper (Capsicum annuum L.) from 6 states of India was assessed. All isolates of R. solanacearum belonged to biovar 3, race 1 and phylotype I. These isolates consisted of 4 distinct DNA types at 75% similarity coefficient using ERIC, BOX and REP-PCRs techniques. Multilocus sequence analysis of hrpB, fliC and egl genes of 6 isolates of R. solanacearum along with 2 out group bacteria was done and they showed high level of variability within these three regions of the genome involving in pathogenicity. |
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| Keywords: | Bacterial wilt biovar Rep-PCR virulence genes phylotyping race |
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