Lindane degradation by root epiphytic bacterium Achromobacter sp. strain A3 from Acorus calamus and characterization of associated proteins |
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Authors: | Tanvi Singh Dileep K. Singh |
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Affiliation: | 1. Department of Zoology, University of Delhi, Delhi, Indiatanvi2112@gmail.com;3. Department of Zoology, University of Delhi, Delhi, India |
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Abstract: | AbstractLindane degrading root epiphytic bacteria were isolated from wetland plant Acorus calamus. Bacterial strain A3 identified as Achromobacter sp. A3, showed maximum degradation potential of 88.7?±?1.24% for 50?mg?l?1 lindane. Lindane biodegradation was followed by decrease in pH as well as increase in concentration of chloride ions in the culture medium. Lindane degradation potential of Achromobacter sp. A3 was also studied at different concentrations of lindane. Maximum degradation was at 10?mg l?1 followed by 50?mg l?1 and 100?mg l?1 lindane. Also, lindane induced proteins were studied using SDS-PAGE. The induced proteins were identified as alpha/beta hydrolase fold-3 domain-containing protein, involved in lindane hydrolysis and extracellular solute-binding family protein having role in transmembrane transport of lindane for utilization of lindane by bacteria. The appearance of unique polypeptides in lane corresponding to media supplemented with lindane showed that the exposure of bacterial cells to lindane has resulted in regulative expression of certain proteins. So far as known, this is the first report to isolate and study lindane degrading root epiphytic bacteria from A. calamus. |
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Keywords: | Acorus calamus Achromobacter sp. A3 Lindane root epiphytic bacteria SDS-PAGE |
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