| 用人胚肺二倍体细胞(2BS和SL7)分离甲型肝炎病毒 |
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| 引用本文： | 焦永真,邵焕英,王宪明,刁武萍,王菊萍,刘晶,陈美微,王勇,佟永力,柯世荣,吴玉花. 用人胚肺二倍体细胞(2BS和SL7)分离甲型肝炎病毒[J]. 病毒学报, 1988, 0(2) |
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| 作者姓名： | 焦永真 邵焕英 王宪明 刁武萍 王菊萍 刘晶 陈美微 王勇 佟永力 柯世荣 吴玉花 |
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| 作者单位： | 卫生部上海生物制品研究所(焦永真，邵焕英，王宪明，刁武萍，王菊萍，刘晶)，黑龙江省卫生防疫站(陈美微，王勇，佟永力，柯世荣)，黑龙江省卫生防疫站(吴玉花) |
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| 摘 要： | 用我国建株的两株人胚肺二倍体细胞(2BS和SL7),以35℃为培养温度,直接从急性期甲型肝炎(简称甲肝)患者粪便中分离到4株病毒。该病毒在细胞培养上符合甲肝病毒的增殖特性,经免疫荧光阻断、免疫电镜及中和试验等特异性鉴定,证实为甲肝病毒。原始分离时的比较研究还显示,2BS株人二倍体细胞支持甲肝病毒增殖的能力似较SL7细胞佳。该4株病毒现已在2BS细胞稳定传代,感染滴度较高,被用于减毒活疫苗的育种研究。
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| 关 键 词： | 甲型肝炎病毒 人二倍体细胞培养 活疫苗 |
PRIMARY ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN 2BS AND SL7 HUMAN DIPLOID CELL CULTURES |
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| Jiao Tongzhen Shao Huanying Wang Xianmin Diao WupingWang Juping Liu Jing Chen Meicheng Wang YongDong Yongli He Shirong Wu Yuhua. PRIMARY ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN 2BS AND SL7 HUMAN DIPLOID CELL CULTURES[J]. Chinese journal of virology, 1988, 0(2) |
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| Authors: | Jiao Tongzhen Shao Huanying Wang Xianmin Diao WupingWang Juping Liu Jing Chen Meicheng Wang YongDong Yongli He Shirong Wu Yuhua |
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| Affiliation: | Jiao Tongzhen Shao Huanying Wang Xianmin Diao WupingWang Juping Liu Jing Chen Meicheng Wang YongDong Yongli He Shirong Wu Yuhua Shanghai Institute of Biological ProductsSanitation and EPidemic Prevention Station of Heilangjiang Province |
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| Abstract: | |
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| Keywords: | HAV Human diploid cell cultures (2BS and SL7)Hepatitis A attenuated vaccine |
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