Transfection of Vibrio cholerae by bacteriophage phi 149 DNA |
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Authors: | M Balganesh J Das |
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Affiliation: | Department of Biophysics Indian Institute of Experimental Medicine Calcutta — 700 032. India |
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Abstract: | DNA isolated from Choleraphage ø149 of Group IV was infectious when mixed with competent cells. The cells were competent during mid-log phase of growth. The infectivity of phage DNA was destroyed by deoxyribonuclease but not by ribonuclease or pronase. About 5 min is required for the establishment of the DNase resistant state. The dose response curve for transfection suggested that 2 to 3 molecules of DNA are required to produce one infections center. An infectivity of 5 × 104 infectious center per μg of DNA was obtained. |
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Keywords: | To whom correspondence should be addressed. |
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