Disulfide bridges of bovine spleen cathepsin B |
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| Authors: | M Baudys B Meloun T Gan-Erdene J Pohl V Kostka |
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| Institution: | Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, CSFR. |
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| Abstract: | Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252. |
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