Production of surfactant and detergent-stable,halophilic, and alkalitolerant alpha-amylase by a moderately halophilic Bacillus sp. Strain TSCVKK |
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Authors: | Kondepudi Kanthi Kiran T. S. Chandra |
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Affiliation: | (1) Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600 036, India;(2) Present address: Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 600 036, India |
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Abstract: | A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase production were optimized with respect to NaCl, substrate, pH, and temperature. Maximum amylase production of 592 mU/ml was achieved in the medium at 48 h with 10% NaCl, 1% dextrin, 0.4% yeast extract, 0.2% tryptone, and 0.2% CaCl2 at pH 8.0 at 30 °C. The enzyme activity in the culture supernatant was highest with 10% NaCl at pH 7.5 and 55 °C. The amylase that was partially purified by acetone precipitation was highly stable in various surfactants and detergents. Glucose, maltose, and maltooligosaccharides were the main end products of starch hydrolysis indicating that it is an alpha-amylase. |
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Keywords: | Moderate halophile Bacillus sp. Strain TSCVKK Alpha-amylase Alkalitolerant Surfactant stable Detergent stable |
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