Carrier-free 8-azidoadenosine 5'-[gamma-32P]triphosphate |
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| Authors: | G P Sabbatini C von Holt |
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| Institution: | UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Republic of South Africa. |
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| Abstract: | We found 8-azidoadenosine 5'-diphosphate to be a phosphoryl acceptor in the enzymatic conversion of 1,3-diphosphoglyceric acid to 3-phosphoglycerate. This has allowed us to synthesize in a single-step procedure carrier-free 8-azidoadenosine 5'-gamma-32P]triphosphate, requiring no further purification of the end product. The synthesized 8-azidoadenosine 5'-gamma-32P]triphosphate has been characterized and shown to meet all the criteria for a specific photoreactive ATP analogue. |
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