Tx基因与Igk基因的同源性研究及其在不同细胞株的表达

本文对以前报道的Ｔｘ基因２．８ｋｂ片段的核苷酸序列与人免疫球蛋白ｋａｐｐａ链Ｃ区基因的核苷酸序列及其编码产物的氨基酸序列进行了同源性比较。结果表明，Ｔｘ基因与ｋａｐｐａ链Ｃ区基因的同源性高达９９．５％以上，编码区的同源性高达１００％。从而提示Ｔｘ基因与ｋａｐｐａ链Ｃ区基因可能是同一种基因。限制性内切酶图谱及Ｓｏｕｔｈｅｒｎ印迹杂交分析，也进一步支持这一观点。本文还报道了ｋａｐｐａ链Ｃ区基因在不同细

Comparison of NPC Transforming Gene Tx to Ig Kappa Constant Region Gene and Their Expressions in Different Cell Lines

We have compared the nucleotide sequence and amino acid sequence of coding product of human immunoglobulin light chain kappa constant region gene(IGKC) to those of human nasopharyngeal carcinoma(NPO) transforming gene(Tx gene)2.8kb EcoR I fragment.The results show that the homology of these two genes is as high as 99.5% and 100 % in coding region, indicating that Tx gene and kappa gene are the same gene.This conclusion is also supported by restriction mapping and Southern blot analysis of the two genes. Meanwhile, the expressions of Tx gene and kappa gene in different malignant cell lines have been investigated. Interestingly,the kappa gene is not only expressed in B lymphatic cell lines(RAji,B95-8, BJAB),but also expressed in NPO cell lines(CNE2,CNE1,HNE1).The expression of this gene has not been detected in human T lymphatic cell lines(Hut 102,MT 2) and in human cervical cell lines(HT-3,ME-180,Hela).The relationship between the expression of kappa gene and NPC carcinogenesis is being investigated.