Protein homeostasis,regulation of energy production and activation of DNA damage-repair pathways are involved in the heat stress response of Pseudogymnoascus spp. |
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| Authors: | Nurlizah Abu Bakar Benjamin Yii Chung Lau Jerzy Smykla Saiful Anuar Karsani Siti Aisyah Alias |
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| Affiliation: | 1. Institute of Ocean and Earth Sciences, C308, Institute of Advanced Studies Building, Universiti Malaya, Kuala Lumpur, 50603 Malaysia;2. Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, Kajang, Selangor, 43000 Malaysia;3. Department of Biodiversity, Institute of Nature Conservation, Polish Academy of Sciences, Mickiewicza 33, Krakow, 31-120 Poland;4. Faculty of Science, Institute of Biological Sciences, Universiti Malaya, Kuala Lumpur, 50603 Malaysia |
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| Abstract: | Proteome changes can be used as an instrument to measure the effects of climate change, predict the possible future state of an ecosystem and the direction in which is headed. In this study, proteomic and gene ontology functional enrichment analysis of six Pseudogymnoascus spp. isolated from various global biogeographical regions were carried out to determine their response to heat stress. In total, 2122 proteins were identified with high confidence. Comparative quantitative analysis showed that changes in proteome profiles varied greatly between isolates from different biogeographical regions. Although the identities of the proteins that changed varied between the different regions, the functions they governed were similar. Gene ontology analysis showed enrichment of proteins involved in multiple protective mechanisms, including the modulation of protein homeostasis, regulation of energy production and activation of DNA damage and repair pathways. Our proteomic analysis did not show any clear relationship between protein changes and the strains' biogeographical origins. |
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