Effects of ultraviolet-B irradiation on the cuticular wax of cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis>) cotyledons |
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Authors: | Satoshi Fukuda Atsushi Satoh Hirokazu Kasahara Hidetoshi Matsuyama Yuichi Takeuchi |
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Institution: | (1) Course of Environmental and Biological Sciences, Graduate School of Science and Engineering, Hokkaido Tokai University, 5-1-1 Minami-sawa, Minami-ku, Sapporo 005-8601, Japan;(2) Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, 5-1-1 Minami-sawa, Minami-ku, Sapporo 005-8601, Japan |
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Abstract: | Cucumber seedlings were grown under three doses of supplemental ultraviolet-B (UV-B) irradiation to examine the effects on
the surface structure of the cotyledons. Medium and high doses of irradiation induced glazing (formation of translucent, glossy
layers) on the adaxial surfaces of cotyledons, especially those exposed to a high dose of UV-B. Observation with a scanning
electron microscope revealed that the adaxial surfaces of cotyledons exposed to a medium dose of UV-B and controls became
rough in appearance, but unevenness of the surface was not apparent in cotyledons irradiated with a high dose of UV-B. UV-B
irradiation affected the types and amounts of alkanes and primary alcohols, the main components of cucumber cuticular wax.
Based on cotyledon area, the amounts of these components were significantly higher in cotyledons irradiated with a medium
dose of UV-B than in controls. This effect could be a consequence of small cotyledon area and constant wax production in the
cotyledons irradiated with a medium dose of UV-B. The distribution patterns of homologs within the alkane and primary alcohol
fractions shifted during growth to longer alkyl chain length in the control cotyledons. UV-B irradiation repressed these changes,
suggesting that UV-B acts on cuticular wax biosynthetic pathways. |
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Keywords: | Cucumber Cuticular wax Epidermis Surface structure UV-B |
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