Digoxigenin-labeled probe for rapid identification of nisinogenic Lactococcus lactis strains |
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Authors: | Jamal Meghrousa Ismail Flissa Mohamed Bouksaima Christophe Lacroixa |
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Institution: | Centre de Recherche en Sciences et Technologie du Lait (STELA), Pavillon Paul-Comtois, UniversitéLaval, Sainte-Foy, Que. G1K 7P4, Canada |
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Abstract: | From the nisZ gene sequence, a non-radioactive digoxigenin-labeled DNA probe, was tested for detection of nisin-producing strains using polymerase chain reaction amplification. The digoxigenin-labeled DNA probe clearly discriminated between nisin-producing and non-producing strains with a high degree of sensitivity and specificity. By agarose gel electrophoresis, 1.4 ng of nisin DNA was detected using the digoxigenin-labeled DNA probe compared with 11 ng using direct polymerase chain reaction amplification. A colony hybridization method using digoxigenin-labeled DNA to selectively detect nisinogenic bacteria showed that the nis-probe was specific and did not react with any other non-bacteriocinogenic and non-nisinogenic strains. |
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Keywords: | Lactococcus strain Digoxigenin-labeled probe PCR Nisin DNA hybridization |
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